FRS Pioneer
|
The first member of our FRS family, FRS™ Pioneer is a chemically defined, animal origin free alternative to foetal bovine serum (FBS) for broad-spectrum cell culture.
FRS™ Pioneer can be added to cell culture media in place of FBS to culture or cryopreserve cells without the use of any animal components or serum (i.e. 100mL of FRS™ Pioneer is equivalent to 100mL of FBS). This allows for reproducible cell culture and provides a cleaner background. To date, analyses indicate that intracellular signalling and cellular function are maintained between FBS and FRS™ Pioneer-cultured cells. SKU: FRSPI0100, FRSPI0500 or FRSPI1000 All prices are in AUD and exclude GST. Enterprise or bulk pricing available upon request.
|
|
*Even with the full knowledge of the reproducibility, regulatory, and sustainability/ethical benefits associated with going serum-free, we know changing scientific processes can be intimidating. FRS Pioneer is broad-spectrum and designed to be readily inserted into your usual FBS-dependent workflows as smoothly as possible. To make it even easier, our scientific co-founder Katie offers personalised support to customers, to ensure your unique culture system's transition away from serum is smooth.
FRS Pioneer has successfully been used by external scientists to achieve:
1. Routine expansion of a broad range of immortalised cell lines (HEK, CHO, JURKAT, MCF7, A549, and many more)
2. Cryopreservation when mixed with 10% DMSO
3. Cleaner assays (e.g. reduced cytokine background)
4. Cell growth in microcarrier systems and on scaffolds
5. Transfection of cell lines for recombinant protein production
6. Exosome research and manufacturing - no extracellular vesicle depletion necessary
7. Reduction of FBS usage to 1% in nutritionally demanding primary and organoid systems
8. Optimised, serum-free media for primary cells, by adding additional cell-specific needs to FRS Pioneer (see datasheets)
And many more applications. Please reach out directly if there is a specific application or use case you're interested in.
2. Cryopreservation when mixed with 10% DMSO
3. Cleaner assays (e.g. reduced cytokine background)
4. Cell growth in microcarrier systems and on scaffolds
5. Transfection of cell lines for recombinant protein production
6. Exosome research and manufacturing - no extracellular vesicle depletion necessary
7. Reduction of FBS usage to 1% in nutritionally demanding primary and organoid systems
8. Optimised, serum-free media for primary cells, by adding additional cell-specific needs to FRS Pioneer (see datasheets)
And many more applications. Please reach out directly if there is a specific application or use case you're interested in.
Product Information
|
FRS Pioneer is: 1. Drop in - an easy substitute for FBS in your laboratory processes 2. Broad spectrum - grows a wide range of immortalised cell lines 3. Chemically defined - ensuring batch consistency and research reproducibility, giving scientists greater experimental control and allowing for background-free, clean interpretation of signalling and functional data. 4. Contaminant free - defined inputs align with regulatory demands and simplify downstream planning for clinical settings 5. Cost-effective - priced on par with Premium FBS 6. Animal origin free - an ethical, sustainable solution free of animal-derived components and associated supply volatility |
|
See how FRS Pioneer performs (data sheets)
Immortalised cell lines
Primary cells
Cryopreservation
Immortalised cell lines
Primary cells
- Growing primary cells with FRS Pioneer - an overview
- Growing adipose-derived mesenchymal stem cells (AD-MSCs) with FRS Pioneer
- Growing chondrocytes with FRS Pioneer
- Growing muscle stem cells (MuSCs) with FRS Pioneer
Cryopreservation
FRS Pioneer User Information and Application Notes
FRS Usage
FRS™ Pioneer is for research use or further manufacturing, not for diagnostic or clinical use.
Safety Data Sheet (SDS)
FRS Pioneer SDS
- FRS Pioneer Quick Start Guide
- White Paper - Transitioning immortalised cell lines to chemically defined, serum free cell culture media with FRS Pioneer
- FRS Pioneer User Guide and Application Notes
FRS Usage
FRS™ Pioneer is for research use or further manufacturing, not for diagnostic or clinical use.
Safety Data Sheet (SDS)
FRS Pioneer SDS
Adhesion and cell attachment
FRS™ Pioneer does not contain adhesion factors found in serum.
For adherent cells, attachment is handled modularly via surface choice or coatings. This allows users to select adhesion strategies appropriate to their system.
The following strategies have been effectively used with FRS Pioneer to facilitate attachment:
Coating choice can influence attachment efficiency and achievable cell density.
For adherent cells, attachment is handled modularly via surface choice or coatings. This allows users to select adhesion strategies appropriate to their system.
The following strategies have been effectively used with FRS Pioneer to facilitate attachment:
- Coating cell culture plates with serum-containing media
- Cell-specific coatings such as vitronectin, fibronectin, laminin, collagen, gelatine, or Matrigel
- Coating with GECKO™, a broad-spectrum, animal-free and chemically defined coating developed by Media City Scientific
- Coating-free workflows in which adherence proteins are added directly to the media (please contact us if this is of interest).
Coating choice can influence attachment efficiency and achievable cell density.
