FRS Pioneer

Across tested immortalised cell lines, FRS™ Pioneer supports:
  • Long-term maintenance across multiple passages
  • Doubling times typically within the 18–30 hour range, depending on cell line and conditions
  • Comparable morphology to FBS controls under appropriate attachment conditions
  • the maintenance of intracellular signalling and cellular function (based on analyses to date)
  • fully animal component free cryopreservation workflows
​Performance is influenced by:
  • Cell type
  • Attachment strategy
  • Seeding density
  • Basal medium choice
Datasheets
​​These datasheets summarise validated performance of FRS Pioneer across common use cases. Results reflect external testing under defined conditions and should be interpreted in the context of your system.​​
  • Immortalised cell lines datasheet: Growth and doubling time comparisons across multiple immortalised cell lines cultured with FRS™ Pioneer versus FBS. 
  • Cryopreservation datasheet: Post-thaw viability and recovery when FRS™ Pioneer is used with 10% DMSO as a freezing medium. 
  • Primary cells datasheet: Short-term support and low-background culture performance for nutritionally demanding primary cell systems, including low-serum workflows. 
​Cell types and use cases
FRS Pioneer has been evaluated primarily on:
  • Immortalised adherent cell lines
  • Immortalised suspension cell lines
  • Routine maintenance workflows
  • Method development and assay setup
It is not currently positioned as a drop-in solution for:
  • Primary cells without optimisation
  • Applications requiring maximum achievable cell density
User information and application notes
Detailed guidance for implementing FRS™ Pioneer in standard laboratory workflows.
 Usage and regulatory information
  • FRS™ Pioneer is supplied for research use or further manufacturing. It is not intended for diagnostic or clinical use.
  • Safety Data Sheet (SDS): Chemical safety, handling, storage, and disposal information. 
  • Sales agreement: Media City Scientific terms of sale. 

GECKO

​GECKO is a chemically defined, animal component–free surface coating designed to support attachment of many adherent cell types to standard tissue culture plastic.
It is intended for use with serum-free or chemically defined media, including FRS™ Pioneer, where adhesion is not provided by serum-derived components.
​What GECKO™ is
GECKO™ is a defined mixture of solubilised adhesion-associated proteins commonly present in FBS, formulated as a ready-to-use surface coating.
GECKO™:
  • Is chemically defined
  • Contains no animal-derived components
  • Is compatible with standard TC–treated plasticware
  • Is designed for broad, general-purpose use.
  • Allows for improved attachment and spreading relative to single-protein coatings for some cell lines.
It is supplied as a coating reagent and does not alter the composition of the culture medium itself.
High level cell line guidance
Attachment performance is cell-type dependent. FRS Pioneer is compatible with standard coatings you may already be using for your cells (e.g. laminin, collagen, gelatine, matrigel).

If you typically culture cells in FBS with no coating, you may:
  • Coat your plates with 10% serum-containing media
  • Coat your plates with chemically defined proteins,

To achieve a fully animal-component-free workflow, either GECKO or recombinant vitronectin alone is typically effective. A first pass recommendation is provided below but please reach out for personalised support.
What GECKO™ is not
GECKO™ is not:
  • A universal adhesion solution for all adherent cell types
  • A guarantee of optimal attachment for every cell line
  • A serum equivalent
Some cell lines have specific needs, often documented in the literature, that are required to achieve reliable attachment, morphology, or downstream performance.

​In these cases, GECKO™ may be suboptimal or inappropriate.
This is expected for any defined coating system.
Datasheets and application notes
GECKO Product Sheet

Usage and regulatory information​
  • GECKO is supplied for research use or further manufacturing. It is not intended for diagnostic or clinical use.
  • Safety Data Sheet (SDS): Chemical safety, handling, storage, and disposal information. 
  • Sales agreement: Media City Scientific terms of sale. 
 GECKO (fibronectin-heavy)
Non-cancer epithelial (CHO, HEK)
Low TGFb cancer (MCF7)
​Mesenchymal (NIH-3T3)
Glioblastoma (U87, A172)
Renal carcinoma (ACHN)
Muscle (C2C12)
Skin (B16-F10)
Vitronectin alone
High TGFb cancer (A549, H460, SW480, DU145)
Hepatocyte (Hep2G)
Normal kidney (MDCK)
Adenocarcinoma (HT-29)
Need help interpreting the data?
If you are unsure how the data applies to your system, optional technical support is available to help you assess fit before or after purchase.

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