Media City Scientific’s Fetal Bovine Serum Replacement Solution (FRS™) Pioneer is a drop-in chemically defined and animal component free FBS replacement. FRS™ Pioneer supports the growth of a broad range of cell lines but can also be substituted for FBS for cell cryopreservation. Mix 10% DMSO with 90% FRS™ Pioneer for broad-spectrum cryopreservation efficacy in the liquid or vapor phase of nitrogen.

FRS™ Pioneer is a drop-in, chemically defined FBS replacement that is:
> Cost effective – priced on par with premium FBS
> Consistent – enabling scientific reproducibility and reliability
> Animal-free – contains no animal derived components
> Effective – supports a broad range of cells at a similar level of performance to FBS

FRS Pioneer versus FBS for cell cryopreservation

Cells were frozen using standard methods in freeze media containing 10% DMSO with either 90% FBS or 90% FRS™ Pioneer. Cells were stored in a liquid or vapor phase nitrogen tank for a period of 1 week to 6 months. Cells were thawed using standard methods. Viability, proliferation, and function were analyzed. Data indicate that, similar to FBS, FRS™ Pioneer is an effective cryopreservation agent when mixed with 10% DMSO. Data sets were collected internally and externally.

Immortalized Cell lines
Tested: A549, CHO, MCF7

Growth of A549 cells following cryopreservation in FBS (red) or FRS™ Pioneer (green) -containing freeze media. (3 months, LN2)
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Primary Cells
Tested: Muscle stem cells, chondrocytes, fibroblasts

Viability of primary fibroblasts following cryopreservation in FBS (red) or FRS™ Pioneer (green) -containing freeze media (6 months, LN2

External Case Study: PBMCs cryopreserved in vapor-phase N2 for 5-14 days using FRS™ Pioneer-containing freeze media retain viability and effector functions following thaw. Long-term biobanking study is in progress. Reach out for more details.
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